Response of industrial hemp to Salsola kali subsp. tragus densities under organic and conventional fertilisation

A 2-year field study was conducted to determine the effects of different nutrition systems and Salsola kali subsp. tragus densities on industrial hemp yield and its components. Experiments were conducted as a split plot in a randomised complete block design with three replicates. Factors were fertilisation (poultry manure, vermicompost and nitrogen fertiliser) as the main plot and weed density (0, 5, 10, 15 and 20 plants/m²) as the subplot. Results indicated that the maximum number of racemes per plant, seeds per raceme, 1000-seed weight, biomass, grain yield and harvest index (HI) of industrial hemp were obtained using vermicompost and were reduced with nitrogen fertiliser application. All measured properties in hemp plants decreased due to increasing S. kali subsp. tragus density, especially at 15 and 20 plants/m². The most significant reduction in hemp grain yield and its components was observed by increasing weed density with the application of nitrogen fertiliser. The application of vermicompost at high weed densities resulted in greater hemp grain yield and its components compared with nitrogen fertiliser. It is recommended to apply organic fertilisers, especially vermicompost at high S. kali subsp. tragus densities.     

不同密度紧凑型油菜的源库特征及与收获指数的相关研究

旨在为油菜株型育种和机械化栽培提供理论和技术依据。选用3个不同类型的紧凑型油菜常规品种,以松散型油菜杂交种‘秦优7号’为对照,研究了在15万、30万、45万、60万、75万、90万株/hm²密度下各品系的库源特征及其与收获指数的关系。结果表明,紧凑型油菜单株在不同密度下的库源特征与对照松散型相同,单株根、茎、枝、壳干重,角粒数和单株角果数随种植密度的增加而降低,且低密度显著高于高密度;千粒重随密度的变化不大。紧凑型油菜的生物学产量和经济产量随种植密度的增加而增加,松散型油菜随种植密度的增加而下降。说明紧凑型油菜适宜密植和机械化收获。收获指数对密度的响应在两类型品种表现一致,最低密度15万株/hm²增加到30万株/hm²时达到峰值,以后随着种植密度的继续增加而降低。收获指数与茎+枝+壳干重之和,与库性状角粒数、角果数、千粒重、单株产量,与库源关系的粒根比、粒茎比、粒枝比、粒壳比显著正相关,说明要提高收获指数不仅要扩“源”增“库”,还要协调库源的合理分配比例。     

太阳能暗管排水对银北灌区油葵土壤环境及产量影响

针对土壤盐渍化严重影响了宁夏银北灌区土壤环境和作物产量的问题,通过太阳能暗管排水区和非暗管排水区对比试验方法,着重研究了2017和2018两年太阳能暗管长时间持续排水对土壤环境和油葵产量的影响。结果表明:太阳能暗管排水可以有效改善土壤环境,使两年的地下水平均埋深分别增加4.5%和6.4%,地下水平均矿化度分别降低7.9%和9.0%,两年土壤平均脱盐率分别为4.7%和8.2%,对表层土壤的脱盐效果最为明显。同时提高了油葵产量和水分生产效率,两年油葵产量分别提高13.8%和21.6%,灌溉水分生产效率分别增加13.3%和21.8%,作物水分生产效率分别增加16.4%和22.9%。综合试验结果和经济成本考虑,油葵生育期灌水2次、播前灌水1次、冬灌1次,生育期5-9月持续排水,这是适宜惠农当地太阳能暗管排水条件下油葵的灌排制度。     

饲料脂肪源和碘酸钾对中华鳖稚鳖生长、存活率、肝肠组织形态和脂类代谢的影响

为了比较饲料中添加鱼油、茶籽油、亚麻籽油以及碘酸钾对中华鳖（）稚鳖生长和脂类代谢的影响，本研究配制了4种油脂含量为10%的饲料，分别为鱼油料（FO，对照组）、鱼油+碘酸钾料（FO+PI，碘酸钾添加量为75 mg/kg）、茶籽油料（TO）和亚麻籽油料（LO），饲喂初重为（5.06±0.05）g的中华鳖66 d。FO+PI组和LO组中华鳖稚鳖的存活率显著低于FO组（<0.05）。各组中华鳖稚鳖血浆中葡萄糖、总胆固醇和甘油三酯水平无显著差异（ACACA）的表达水平，上调了中华鳖-499的表达水平（-23b的表达水平（<0.05）。FO+PI组和TO组的稚鳖肝脏细胞内脂滴空泡较少，同时TO显著影响了肠道组织的黏膜褶。结论认为，相比于鱼油组，在饲料中添加10%茶籽油、亚麻籽油和添加75 mg/kg碘酸钾不会引起稚鳖生长差异，但是亚麻籽油和碘酸钾降低了稚鳖的存活率，茶籽油和碘酸钾影响肝脏的脂类代谢。     

植物源蒸馏油对柑橘木虱控制效果的评价

植物源蒸馏油是一种新型植物油，本文探讨了植物源蒸馏油在害虫控制中的应用潜力。以矿物油乳剂为参照，以柑橘木虱为试虫，分析比较植物油蒸馏油的触杀和拒避作用。植物油和矿物油对柑橘木虱的卵、若虫和成虫均有一定的触杀作用，其中对若虫尤其低龄若虫的触杀作用最强，可达90%以上。两种油剂对雌虫产卵均有一定的拒避作用，拒避雌虫产卵维持约2 d。两种油剂对成虫寄主选择没有明显的拒避作用。总体上，植物源蒸馏油对柑橘木虱的触杀作用以及对成虫的拒避作用与矿物油相当。如果进一步改进该植物油的乳化效果，可替代矿物油乳剂应用于害虫防治中。     

中国古代油料与油脂研究综述

油脂分为动物脂肪与植物油脂,油脂不仅是人类的主要营养品和食物之一,也是重要的生产资料。人类很早就利用油脂满足各种生活需要。动物脂肪首先被广泛应用于烹饪、照明、润滑、仪式活动、艺术等方面。汉代以来,随着油料作物的广泛种植,植物油品种不断增多,生活用油更加多元化。中国古人非常重视油料作物的栽培,在榨油工具方面形成了具有本土特色的多元化的生产技艺,油脂的应用也深入到了生活的方方面面。本文梳理并总结目前古代油脂研究的成果,对减少油料进口、实现粮食供给安全,以及非物质文化遗产的保护等,都具有重要意义。     

甘蓝型油菜耐低温生长生理生化响应机制

为提高甘蓝型油菜耐寒育种过程中的筛选效率，研究甘蓝型油菜耐低温机理，以8个不同抗寒性的甘蓝型油菜为材料，对低温条件下各材料的生物量、叶绿素、脯氨酸和相对电导率进行测定，发现在室外平均气温2.75℃时，抗性材料的生物量、叶绿素和脯氨酸的累积量都显著高于敏感材料，相对电导率没有显著差异；当平均气温7.52℃回升至12.39℃，抗性材料和敏感材料生物量的累积量无显著差异；在恒定低温10℃/4℃处理下抗性材料和敏感材料在处理前3周生物量均持续累积，但从第4周开始敏感材料新叶出现白斑，生物量减少，抗性材料老叶出现白斑，生物量维持不变。结果表明，耐低温材料在低温条件下叶绿素含量受到的影响较小，脯氨酸的积累量持续上升，具有较强的快速适应能力，在低温下具有显著的持续生长优势。     

Characterization of Genomic Integration and Transgene Organization in Six Transgenic Rapeseed Events

To characterize the DNA rearrangement of both the T-DNA region and the genomic insertion site during T-DNA insertion, the Genomewalker strategy was used to isolate the junctions between the inserted DNA and the plant genomic DNA in six rapeseed events as well as the genomic DNA at the sites before integration. During transformation in each of the six events, portions of both the right border（RB） and left border（LB） regions of the T-DNA were deleted, ranging from a 7 nucleotide deletion of the LB repeats in event RF1 to a 207 bp deletion of the LB region in event RF2. For the six events, T-DNA integration resulted in a deletion at the target site spanning less than 100 bp. Sequence analysis indicated that the T-DNA was integrated into the coding region of various native rapeseed genes in events RF1 and RF2. Duplications of the genomic DNA target site were observed in events RF2, RF3 and Topas 19/2. And multimerization of transgenes was found in event Topas 19/2, in which, the T-DNA was integrated as a head-to-head（RB-to-RB） concatemer into the recipient genome. In event MS1, chromosomal translocation or a large target-site deletion may have occurred during T-DNA integration, which was identified due to a failure to amplify the presumptive insertion site based on the flanking rapeseed DNA sequences. Our results provide comprehensive data concerning transgene organization and the genomic context of the T-DNA in six rapeseed events, which can aid in the developing of insert fingerprinting and the monitoring of long-term genetic stability and potential unintended effects of transgenic events.     

柞蚕雄蛾油对大鼠辅助降血脂功能的影响研究

为研究柞蚕雄蛾油对小鼠血脂代谢的影响,进行了雄性Wistar大鼠高脂饲料与不同剂量柞蚕雄蛾油的喂养试验,每4天称体重一次,每4天测定小鼠肝系数及血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL‐C）和低密度脂蛋白胆固醇（LDL‐C）一次。结果表明：3个剂量组TC、TG、LDL‐C含量与高脂模型组比较显著低于高脂模型组,而HDL‐C显著高于模型组,具有显著性差异（P＜0．05）。